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Separation of pure toxic peptides from a β-gliadin subfraction using high-performance liquid chromatography

Clinica Chimica Acta, ISSN: 0009-8981, Vol: 134, Issue: 1, Page: 189-198
1983
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Article Description

The β v subfraction was isolated from peptic-tryptic digests of β-gliadin by chromatography on Biogel P-10 and applied to a Lichrosorb RP-18 or a μ-Bondapak C-18 column. Fractionation was achieved using reverse-phase high-performance liquid chromatography with a linear gradient of acetonitrile in ammonium acetate. A better resolution was obtained with the μ-Bondapak column.

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