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Isolation of thyroid peroxidase from patients with graves' disease and comparison with animal peroxidases

International Journal of Biochemistry, ISSN: 0020-711X, Vol: 26, Issue: 3, Page: 351-365
1994
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1.1. Human thyroid peroxidase (TPO) was isolated from 280-640 g of pooled thyroid tissue resected from patients with Graves' disease.2.2. Isolation was performed by an improved and simplified method.3.3. The Reinheit Zahl (A412/A280) of the final preparations was in the range of 0.16–0.32.4.4. The spectroscopic and enzymatic properties of Graves' TPO were compared with those of porcine TPO and bovine LPO, revealing closer resemblance to the former.5.5. Graves' TPO may provide a useful substitute for normal TPO, which is very difficult to isolate.

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