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A method to evaluate the biosynthesis of glycosaminoglycans by the aorta of cynomolgus monkey

Atherosclerosis, ISSN: 0021-9150, Vol: 45, Issue: 3, Page: 359-363
1982
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Article Description

The biosynthesis of the glycosaminoglycans (GAGs) was investigated in vitro in the aortic tissue of the Cynomolgus monkey incubated with [ 14 C]glucosamine. With the use of a new micromethod, it was possible to quantify the glycosaminoglycans and their radioactive distribution in the aortic tissue and incubation medium. Labeled and nonlabeled chondroitin 6-sulfate, dermatan sulfate, heparan sulfate and hyaluronic acid were measured. Since the sensitivity of this procedure is between 5 and 20 μg of GAGs, as little as 5 mg of dry defatted aortic tissue is sufficient for chemical and radioactive analyses.

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