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Variations in oxidative susceptibility among six low density lipoprotein subfractions of differing density and particle size

Atherosclerosis, ISSN: 0021-9150, Vol: 93, Issue: 3, Page: 189-199
1992
  • 454
    Citations
  • 0
    Usage
  • 41
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    454
    • Citation Indexes
      454
  • Captures
    41

Article Description

Oxidative modification of low density lipoproteins (LDL) has been implicated in the sequence of events leading to fatty streak formation in the arterial intima. Increased oxidative modifications of dense versus bouyant LDL particles could contribute to increased atherosclerosis associated with lipoprotein profiles enriched in small, dense LDL. In the present studies, we compared rates of copper-induced oxidative changes for six LDL subfractions ranging in density from 1.023 to 1.053 g/ml and mean particle diameter from 282 ± 10 to 245 ± 3. Rates of formation of thiobarbituric acid-reactive substances (TBARS), as indicated by the time required for half-maximal TBARS formation ( T12max ), decreased with increasing density and decreasing particle diameter to a minimum in fraction 5 ( d = 1.046 g/ml, diameter = 250 ± 5) ( P = 0.007). In parallel studies using unfractionated LDL ( d = 1.019–1.063 g/ml), T12max values were inversely correlated with the predominant LDL species diameter as determined by 2–16% gradient gel electrophoresis ( P < 0.05), consistent with the involvement of subclass composition of subclass composition in determining oxidative behavior. In separate experiments, subfraction differences in oxidation rates as assessed by TBARS formation were verified by the finding of similarly disparate changes in fluorescence intensity and anionic electrophoretic mobility. T12max values were not related to LDL contents of alpha-tocopherol, beta-carotene, protein, triglycerides or phospholipids, but were significantly correlated with unesterified cholesterol content ( r = 0.46; P < 0.001) and were inversely associated with cholesterol ester content ( r = 0.28; P < 0.05). The positive association of T12max with unesterified cholesterol suggests that this constituent may impart resistance to oxidative modification, possibly by altering properties of the surface monolayer where it resides.

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