Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran
Journal of Immunological Methods, ISSN: 0022-1759, Vol: 144, Issue: 2, Page: 157-163
1991
- 32Citations
- 10Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations32
- Citation Indexes32
- 32
- CrossRef20
- Captures10
- Readers10
- 10
Article Description
We have sought a transfection strategy which preserves cell viability and achieves sufficient efficiency to perform reporter gene assays in primary cultures of thioglycollate-elicited murine peritoneal macrophages. Murine peritoneal macrophages were transfected with an eukaryotic expression vector containing the Rous sarcoma virus enhancer-promoter upstream of the bacterial chloramphenicol acetyl-transferase reporter gene (FC4-CAT). Transfection using DEAE-dextran followed by 10% DMSO provided much higher CAT activity than either calcium-phosphate or lipofection. The transfected macrophages increased CAT activity (1.9–7.6-fold) following stimulation with 10% serum. DEAE-dextran/DMSO-mediated transfection provides a simple, inexpensive method to transfect primary cultures of adherent macrophages with heterologous plasmid DNA. Transfection of macrophages with a CAT reporter gene using this method permits the characterization of gene regulation in primary macrophage cultures in vitro.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/002217599190082Q; http://dx.doi.org/10.1016/0022-1759(91)90082-q; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025947392&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/1960412; https://linkinghub.elsevier.com/retrieve/pii/002217599190082Q; http://linkinghub.elsevier.com/retrieve/pii/002217599190082Q; http://api.elsevier.com/content/article/PII:002217599190082Q?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:002217599190082Q?httpAccept=text/plain; http://dx.doi.org/10.1016/0022-1759%2891%2990082-q; https://dx.doi.org/10.1016/0022-1759%2891%2990082-q
Elsevier BV
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