EPR study of the oxidation of phenylhydrazine initiated by the e titanous chloride/hydrogen peroxide reaction and by oxyhemoglobin
Journal of Magnetic Resonance (1969), ISSN: 0022-2364, Vol: 65, Issue: 3, Page: 491-496
1985
- 12Citations
- 1Captures
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Article Description
We have studied by EPR spectroscopy at 25°C and over the pH range 5.0–10.0 the substrate phenylhydrazine with the use of the TiCl 3 H 2 0 2 rapid-mixing, continuous-flow, radical-generating method. The complex EPR spectrum observed is fully interpretable in terms of the PhNNHZ radical. So far, there have been no reports in the literature of EPR data for this radical. In addition, we have examined the oxidation of phenylhydrazine by oxyhemoglobin at 25°C at pH 7.4 with the use of a rapid-mixing, continuous-flow method analogous to that employed for the TiC13-H 2 0 2 /phenylhydrazine investigation. Once again, the EPR absorption observed is fully interpretable in terms of PhNNH 2. Comparison of the procedures and results obtained with the use of the two methods suggests that the TiC13-H 2 0 2 /phenylhydrazine and oxyhemoglobin/phenylhydrazine reaction systems are of similar effectiveness in generating PhNNHZ radicals.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0022236485901349; http://dx.doi.org/10.1016/0022-2364(85)90134-9; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=1342300244&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/0022236485901349; https://api.elsevier.com/content/article/PII:0022236485901349?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:0022236485901349?httpAccept=text/plain; http://dx.doi.org/10.1016/0022-2364%2885%2990134-9; https://dx.doi.org/10.1016/0022-2364%2885%2990134-9
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