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Termination of RNA transcription on the replicative form DNA of bacteriophage fd

Journal of Molecular Biology, ISSN: 0022-2836, Vol: 62, Issue: 1, Page: 81-88
1971
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Article Description

In an RNA synthesizing system consisting of the doubly closed replicative form DNA of bacteriophage fd as template and purified Escherichia coli RNA polymerase holoenzyme, synthesis of RNA chains starting with pppA— proceeded to a size corresponding to about one turn of the template and terminated at either low or high salt. Transcription of the (pppA—) RNA chains was restricted to at least five different sites by adding the rho factor. Rho-induced termination at two of these sites was active even at high salt (0.2 m -KCl), whereas the termination at the other sites functioned only at low salt (less than 0.1 m -KCl).

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