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Studies of the binding of Escherichia coli RNA polymerase to DNA

Journal of Molecular Biology, ISSN: 0022-2836, Vol: 70, Issue: 2, Page: 157-185
1972
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Article Description

A membrane filter assay has been devised to study the binding of Escherichia coli RNA polymerase to DNA. RNA polymerase is quantitatively retained on nitrocellulose filters, and DNA bound in complexes between RNA polymerase and labeled T7 DNA is retained with an efficiency of up to 70%. The attachment of a single RNA polymerase molecule to a molecule of T7 DNA is sufficient to cause retention of the DNA molecule on the filter.

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