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Epitope mapping of alpha-transforming growth factor: Evidence of an immunodominant region

Life Sciences, ISSN: 0024-3205, Vol: 42, Issue: 24, Page: 2525-2531
1988
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Article Description

Antisera were produced in rabbits and sheep against both full-length synthetic rat alpha-transforming growth factor and peptides corresponding to the carboxy-terminal 17 amino acids. These antisera were used to develop a peptide based radioimmunoassay of alpha-TGF. All antisera reacted only with a restricted region of the alpha-TGF corresponding to the 8 residues (43–50) at the carboxy-terminus: Cys43 Glu44 His45 Ala46 Asp47 Leu48 Leu49 Ala50

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