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Oxygen affinity of duck blood determined by in vivo and in vitro technique

Respiration Physiology, ISSN: 0034-5687, Vol: 29, Issue: 3, Page: 355-361
1977
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Half saturation partial pressure of O 2, P 50 ). was determined in domestic Muscovy ducks ( Cuirinu moschala ) both by in vivo and in vitro techniques. For in riro determination, blood samples were drawn from the anesthetized, artificially ventilated animals and analyzed both for O 2 content, C o 2 and O 2, partial pressure, P o 2, O 2 ; capacity was determined in arterial samples during hyperoxic ventilation (arterial po, about 180 torr). P 50 was calculated from measurements in venous blood samples (O 2 saturation near 50%). For in vitro determinations, C o 2, was measured in blood samples equilibrated with P o 2 close to P 50. No significant difference was found between P 50 values determined by both techniques. At 41 C and pH 7.50. P 50 averaged 41.7 torr when analyzed by in vivo technique and 41.4 torr when determined in vitro. The variability between animals was less than 1 torr (SD) and could be explained by the experimental error. The partially discordant literature data on P 50 of duck blood are reviewed and critically discussed.

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