Protoplasts of Neurospora crassa: α-amylase improves yield from wild-type hyphae
Experimental Mycology, ISSN: 0147-5975, Vol: 9, Issue: 3, Page: 4-58
1985
- 7Citations
- 2Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations7
- Citation Indexes7
- CrossRef7
- Captures2
- Readers2
Article Description
Treatment of wild-type hyphae of Neurospora crassa with a mixture of chitinase (4 units/ml), Zymolyase 20T (5 units/ml), cellulysin (100 units/ml), lytic enzyme L-1 (1 mg/ml), and β-glucuronidase (1500 units/ml) to which 5000 units/ml α-amylase had been added resulted in the quantitative conversion of hyphae to protoplasts which did not aggregate or clump. Overall, α-amylase addition improved protoplast yield by over twofold.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0147597585900210; http://dx.doi.org/10.1016/0147-5975(85)90021-0; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85058200001&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/0147597585900210; https://api.elsevier.com/content/article/PII:0147597585900210?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:0147597585900210?httpAccept=text/plain; http://dx.doi.org/10.1016/0147-5975%2885%2990021-0; https://dx.doi.org/10.1016/0147-5975%2885%2990021-0
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