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Protoplasts of Neurospora crassa: α-amylase improves yield from wild-type hyphae

Experimental Mycology, ISSN: 0147-5975, Vol: 9, Issue: 3, Page: 4-58
1985
  • 7
    Citations
  • 0
    Usage
  • 2
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    7
    • Citation Indexes
      7
      • CrossRef
        7
  • Captures
    2

Article Description

Treatment of wild-type hyphae of Neurospora crassa with a mixture of chitinase (4 units/ml), Zymolyase 20T (5 units/ml), cellulysin (100 units/ml), lytic enzyme L-1 (1 mg/ml), and β-glucuronidase (1500 units/ml) to which 5000 units/ml α-amylase had been added resulted in the quantitative conversion of hyphae to protoplasts which did not aggregate or clump. Overall, α-amylase addition improved protoplast yield by over twofold.

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