PlumX Metrics
Embed PlumX Metrics

Low level of SO 2 enhanced chromatid aberrations in tradescantia pollen tubes and seasonal variation of the aberration rates

Mutation Research/Environmental Mutagenesis and Related Subjects, ISSN: 0165-1161, Vol: 21, Issue: 2, Page: 93-100
1973
  • 32
    Citations
  • 0
    Usage
  • 1
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    32
    • Citation Indexes
      30
    • Policy Citations
      2
      • Policy Citation
        2
  • Captures
    1

Article Description

Pollen tube cultures of Tradescantia paludosa Anders. Sax clone-3 were grown on microslides coated with lactose agar medium (0.02% colchicine). They were incubated in an air-tight moist chamber for 18 h to obtain the metaphase chromosomes of the pollen tube generative nuclei. SO 2 treatments were applied at the beginning of the germination stage, 7–10 min after sowing the pollen. A total of about 0.075 ppm of SO 2 was used for the entire incubation period. SO 2 gas was either generated by the reaction between K 2 S 2 O 5 and HCl inside the chamber or obtained from diluting the gas from commercial SO 2 gas (Matheson Gas Products) of known concentration through a gas dilution system. Full-grown cultures fixed in Gate's fluid and stained in Feulgen were made into permanent slides for aberration-rate determination. Year-round study of SO 2 effect on chromosomes was made possible by using a GRO-LUX lamp (Sylvania) illuminated incubator, in winter, to promote mitotic activity of pollen culture. In these studies, SO 2 enhanced chromatid aberration rates (average 46 breaks/100 cells) in the cultures grown from field or hot-house plants during 3 consecutive summers (1970–1972). There was a gradual decrease in the fall and a gradual increase in spring in both background and enhanced aberration rates. When summer pollen cultures from GRO-LUX lamp pre-conditioned (7–20 days) plants were treated with SO 2 in the same chamber with those grown from summer hot-house plants, high rate (42 breaks/100 cells) of aberration was observed only in cultures from summer hot-house plants.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know