Calcium uptake and calcium-dependent phosphorylation during development of rat brain neurons in culture
Developmental Brain Research, ISSN: 0165-3806, Vol: 13, Issue: 2, Page: 293-303
1984
- 13Citations
- 3Captures
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Article Description
The emergence of the capacities for calcium uptake and calcium-regulated protein phosphorylation during the development of embryonic brain neurons in tissue culture was examined. In the maturing cells, the enhancement in 45 Ca 2+ -uptake upon stimulation with high K + increased by 3–4 fold during the second week in vitro, in parallel to an increase in the capacity for high K + -induced Ca 2+ -dependent release of prelabeled [ 3 H]dopamine. The pattern of incorporation of [ 32 P i ]phosphate into the major phosphoproteins in maturing cells under nonstimulating conditions also changed during cell development: the incorporation of 32 P i into two proteins of apparent molecular weights — 55,000 and 43,000 dalton — increased, but decreased in a 45,000 dalton protein. Stimulation of mature cells (after 10–11 days in vitro) resulted in a Ca 2+ -dependent increase in the amount of 32 P i incorporated into the 43,000 dalton protein and a decrease in the amount incorporated into the 55,000 dalton protein. This calcium-regulated phosphorylation pattern was not observed until 6 days in vitro. Introduction of Ca 2+ into the immature cells by means of the Ca 2+ ionophore A23187 did not alter the phosphorylation pattern and did not cause neurotransmitter release. The amount of [ 35 S]methionine incorporated into a 43,000 dalton protein which comigrated with the 43,000 dalton phosphoprotein also increased upon cell maturation. The results suggest that this phosphoprotein (which does not comigrate with nonphosphorylated actin on two-dimensional polyacrylamide gels) develops in the cells in parallel to the emerging processes of the stimulation-induced calcium entry and calcium-dependent neurosecretion.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0165380684901640; http://dx.doi.org/10.1016/0165-3806(84)90164-0; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021227693&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/6144371; https://linkinghub.elsevier.com/retrieve/pii/0165380684901640; http://dx.doi.org/10.1016/0165-3806%2884%2990164-0; https://dx.doi.org/10.1016/0165-3806%2884%2990164-0
Elsevier BV
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