Bivariate assay for cleaved atrial natriuretic factor (ANF(101–105/106–126)) in sheep plasma
Regulatory Peptides, ISSN: 0167-0115, Vol: 40, Issue: 3, Page: 389-396
1992
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Article Description
A bivariate assay has been developed to measure both ANF, and cleaved ANF (ANF(101–105/106–126)) the primary product of endopeptidase-24.11 action on rat ANF(101–126). The assay employed two different antisera directed to the carboxy-terminal and ring regions of ANF to enable discrimination and measurement of intact endogenous ANF(99–126) and infused cleaved ANF (rat ANF(101–105/106–126)) in sheep plasma extracts. The assay which was validated by HPLC analysis of plasma extracts, had sufficient accuracy and precision to measure the metabolic clearance rate and half-life of cleaved ANF when infused in normal sheep.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/016701159290525Y; http://dx.doi.org/10.1016/0167-0115(92)90525-y; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026802139&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/1438981; https://linkinghub.elsevier.com/retrieve/pii/016701159290525Y; http://linkinghub.elsevier.com/retrieve/pii/016701159290525Y; http://api.elsevier.com/content/article/PII:016701159290525Y?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:016701159290525Y?httpAccept=text/plain; http://dx.doi.org/10.1016/0167-0115%2892%2990525-y; https://dx.doi.org/10.1016/0167-0115%2892%2990525-y
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