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[ 125 I]Endothelin binding in rat cerebellum is increased following l -2-chloropropionic-acid-induced granule cell necrosis

Peptides, ISSN: 0196-9781, Vol: 16, Issue: 5, Page: 897-902
1995
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The systemic administration of l -2-chloropropionic acid ( l -CPA) to rats produced a marked depletion of cerebellar granule cells (> 80% of the total) when administered in a single oral dose of 750 or 250 mg/kg/day for 3 days. The nature of the l -CPA-induced neurotoxicity is currently unknown but it exhibits a number of features in common with excitatory amino acid-induced neuronal cell death. We observed an increase in [ 125 I]endothelin-1 (ET-1) binding in the cerebellar cortex, as measured by quantitative receptor autoradiography, which occurs at 48 h, but not 24 h, following the 750 mg/kg l -CPA dosing regimen. The increase in [ 125 I]ET-1 binding did not parallel the cellular damage and resultant astrocyte proliferation, as measured by GFAP immunoreactivity, which was primarily confined to the granular layer of the cerebellar cortex. The increased [ 125 I]ET-1 binding occurred in the molecular layer of the cerebellar cortex (controls 2.03 ± 0.26 fmol/mg tissue; l -CPA-treated 6.69 ± 0.45 fmol/mg tissue, n = 6; p < 0.01, Student's t -test) which appeared to contain astrocytic processes originating from the large increase in astrocyte number situated in the granular layer. Pretreatment of the rats with the irreversible NMDA receptor antagonist, MK-801, protected the cerebellar granule cells against the L-CPA neurotoxicity and also prevented the increase in [ 125 I]ET-1 binding in the cerebellar cortex. The increased [ 125 I]ET-1 binding in rat cerebellum appears to be linked to the reactive gliosis that occurs in association with neuronal cell injury.

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