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Molecular characterization of D 2 dopamine-like receptors from brain and from the pituitary gland

Neurochemistry International, ISSN: 0197-0186, Vol: 21, Issue: 2, Page: 159-169
1992
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Article Description

D 2 dopamine-like receptors have been purified from five bovine brain regions (caudate nucleus, putamen, olfactory tubercle, frontal cortex, cerebellum) and the anterior and neurointermediate lobes of the pituitary gland using a combined ligand-affinity and lectin-affinity chromatography procedure. In all the brain regions except cerebellum and in the neurointermediate lobe of the pituitary gland the purified species appeared as a M r 95,000 doublet on SDS-PAGE. In the anterior lobe of the pituitary an additional M r 142,000–145,000 species was seen.

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