Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis
Gene, ISSN: 0378-1119, Vol: 25, Issue: 2, Page: 301-308
1983
- 48Citations
- 10Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations48
- Citation Indexes48
- CrossRef48
- 25
- Captures10
- Readers10
- 10
Article Description
By means of homopolymer dG-dC tailing, using PstI linearized pBR327 as vector, we constructed small plasmids containing the entire Escherichia coli recA gene. The 1.8-kb inserts were recloned in the Bacillus subtilis expression vector pPL608 in a B. subtilis recE 4 strain. Analysis of plasmid-coded proteins showed expression of the E. coli recA gene both in minicells and whole cells of B. subtilis. Expression was under control of the bacteriophage SP02 promoter, which is part of pPL608. A recA -expressing plasmid completely abolished the transformation deficiency of the recE 4 mutant as well as its sensitivity to mitomycin C(MC). The expressed recA gene also restored recombination in other B. subtilis strains lacking the recE gene product. These results indicate a high similarity between the functions of the E. coli RecA and B. subtilis RecE proteins.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0378111983902342; http://dx.doi.org/10.1016/0378-1119(83)90234-2; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021049972&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/6420239; https://linkinghub.elsevier.com/retrieve/pii/0378111983902342; http://dx.doi.org/10.1016/0378-1119%2883%2990234-2; https://dx.doi.org/10.1016/0378-1119%2883%2990234-2
Elsevier BV
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