Expression of a cDNA encoding human 5-lipoxygenase under control of the STA1 promoter in Saccharomyces cerevisiae
Gene, ISSN: 0378-1119, Vol: 89, Issue: 2, Page: 231-237
1990
- 26Citations
- 7Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations26
- Citation Indexes26
- 26
- CrossRef20
- Captures7
- Readers7
Article Description
A yeast-expression vector utilizing the STA1 promoter was constructed, and shown to be useful for the expression of a heterologous gene. A cDNA, encoding human 5-lipoxygenase (5LO), was inserted into the vector and expressed in Saccharomyces cerevisiae. The enzyme (yh5LO) produced in the transformant was purified to homogeneity from the cellular soluble fraction. The purified enzyme showed both 5LO and leukotriene A 4 synthase activities, which were stimulated by Ca 2+ and ATP. The N-terminal end of yh5LO contained five extra amino acids not present in 5LO purified from human leukocytes. A human 5LO-secretion vector containing the STA1 signal sequence was also constructed. When this hybrid gene was expressed in S. cerevisiae, its product was glycosylated and accumulated in the fractions related to the secretory pathway.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/037811199090010O; http://dx.doi.org/10.1016/0378-1119(90)90010-o; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025350532&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/2115489; http://linkinghub.elsevier.com/retrieve/pii/037811199090010O; http://api.elsevier.com/content/article/PII:037811199090010O?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:037811199090010O?httpAccept=text/plain; https://linkinghub.elsevier.com/retrieve/pii/037811199090010O; http://dx.doi.org/10.1016/0378-1119%2890%2990010-o; https://dx.doi.org/10.1016/0378-1119%2890%2990010-o
Elsevier BV
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