PlumX Metrics
Embed PlumX Metrics

Transduction of a signal for arachidonic acid metabolism by untriggered CSF-1 receptor induces an opposite effect to that induced by CSF-1 receptor and its ligand: Separate regulation of phospholipase A 2 and cyclooxygenase by CSF-1 receptor/CSF-1

Prostaglandins, Leukotrienes and Essential Fatty Acids, ISSN: 0952-3278, Vol: 45, Issue: 1, Page: 43-48
1992
  • 3
    Citations
  • 0
    Usage
  • 9
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

The mouse hematopoietic cell line, 32D, was transfected with c-fms, which encodes for the CSF-1 receptor, a tyrosine kinase (TK). In the absence of CSF-1, transfected cells show moderate levels of arachidonic acid (AA) release and produce a substantial amount of prostaglandin E 2 (PGE 2 ) in comparison with the original cell line. Exposure of transfected cells to CSF-1, while inducing a substantial increase in arachidonate release, nevertheless resulted in inhibition of PGE 2 production. Addition of ST638, a tyrosine kinase inhibitor, to cells transfected with c-fms in the absence of CSF-1 inhibited PGE 2 production within 10–60 min. Its addition to the same cells in the presence of CSF-1 induced an opposite effect, but required longer treatment (24 h). In either cell type, AA release was not affected by this agent. These data indicate that CSF-1 may regulate cyclooxygenase activity. The different effect of CSF-1 receptor on PGE 2 production in the presence or absence of CSF-1 and the opposite effect of a tyrosine kinase inhibitor on PGE 2 suggest that both the receptor alone or the receptor-ligand complex may transduce an active, but different, signal through tyrosine phosphorylation. CSF-1 receptor and CSF-1 may exert separate, but related, effects on phospholipase A 2 and cyclooxygenase activity which, in concert, or along with other tyrosine kinases, regulate prostaglandin production.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know