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12 Use of Monoclonal Antibodies to Study Mycorrhiza: Present Applications and Perspectives

Methods in Microbiology, ISSN: 0580-9517, Vol: 24, Issue: C, Page: 221-248
1992
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Article Description

This chapter describes technical steps involved in the production of hybridomas. The chapter outlines those most relevant characteristics of monoclonal antibodies which are important for an understanding of their potential applications to the study of plant-microbe interactions. The main steps in the production of hybridomas and in the selection of clones are described and some practical examples of screening strategies provided. The application of hybridoma technology to the study of mycorrhiza is still very infrequent and the few McAbs so far derived to plant and fungal components are described. Particularly useful, however, is the experience collected in the production of monoclonal antibodies to other types of plant-microbe interactions, and these are highlighted to reveal problems and strategies that may be of a general application. Hybridoma technology is adopted to investigate various aspects of plant differentiation and plant-microbe interactions. The use of monoclonal antibodies has revealed the presence of particular epitopes on the cell surfaces of both plants and micro-organisms whose expression is modulated during development and during the establishment of symbiotic or pathogenic interactions.

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