Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy
Methods in Cell Biology, ISSN: 0091-679X, Vol: 137, Page: 205-224
2017
- 15Citations
- 16Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations15
- Citation Indexes15
- CrossRef15
- 11
- Captures16
- Readers16
- 16
Article Description
The spatiotemporal characteristics of ESCRT (Endosomal Sorting Complex Required for Transport)-mediated mammalian cytokinetic abscission have been studied in recent years using quantitative high-resolution light microscopy techniques. Here we describe how to apply spinning disk live cell imaging and structured illumination microscopy (SIM) to define the dynamics and structural organization of abscission and of proteins involved in abscission in a quantitative manner. We further provide a protocol to correlate the structural data, obtained by SIM, to the dynamic information obtained by live cell recordings.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0091679X16300243; http://dx.doi.org/10.1016/bs.mcb.2016.03.032; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84996843086&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/28065306; https://linkinghub.elsevier.com/retrieve/pii/S0091679X16300243; https://dx.doi.org/10.1016/bs.mcb.2016.03.032
Elsevier BV
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