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In vivo detection and quantification of chemicals that enhance protein stability

Analytical Biochemistry, ISSN: 0003-2697, Vol: 434, Issue: 1, Page: 181-186
2013
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Article Description

We have devised protein-folding sensors that link protein stability to TEM-1 β–lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β–lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.

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