Generation and characterization of avian IgY antibodies for detecting beta-casein A1 in bovine milk
Analytical Biochemistry, ISSN: 0003-2697, Vol: 678, Page: 115283
2023
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Article Description
Beta-casein is a primary milk protein that constitutes approximately 30% of the casein in bovine milk, with the two most common types in cattle being A1 and A2. The A2 protein differs from the A1 version due to a mutation in the codon at position 67, resulting in a histidine to proline substitution. However, the bioactive peptide, beta-casomorphine-7 (BCM7), which originates from partial proteolysis of the A1 variant, has been linked to several gastrointestinal disorders in humans. Production of A1 beta casein-free products is increasing demand in the milk market, worldwide. This study generated and characterized a polyclonal IgY antibody that specifically recognizes the A1 beta-casein protein present in cow's milk. A commercially available IgY anti-A1 antibody was used as a positive control, and the sensitivity and specificity of both the commercial and produced anti-A1 antibodies were evaluated. The results showed 100% sensitivity and specificity of 100% of the commercial IgY anti-A1. The in-house produced anti-A1 antibody demonstrated a sensitivity of 95.2% and a specificity of 100%, indicating its potential as a reliable and cost effective tool for detecting A1 beta-casein protein in milk samples.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0003269723002488; http://dx.doi.org/10.1016/j.ab.2023.115283; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85167991352&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/37572840; https://linkinghub.elsevier.com/retrieve/pii/S0003269723002488; https://dx.doi.org/10.1016/j.ab.2023.115283
Elsevier BV
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