An ionic liquid-assisted sample preparation method for sensitive integral-membrane proteome analysis
Analytical Biochemistry, ISSN: 0003-2697, Vol: 683, Page: 115349
2023
- 2Citations
- 5Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations2
- Citation Indexes2
- Captures5
- Readers5
Article Description
Many ion channels and receptor proteins are potential targets for new drugs. However, standard methods for profiling these integral membrane proteins (IMPs) have not been fully established, especially when applied to rare and quantity-limited biological samples. We previously demonstrated that a mixture containing 1-butyl-3-methylimidazolium cyanate, an ionic liquid (IL), and NaOH (termed i -soln) is an excellent solubilizer for insoluble aggregates. In this study, we present a combined i -soln-assisted proteomic sample preparation platform (termed pTRUST), which is compatible with starting materials in the sub-microgram range, using our previously reported i -soln-based sample preparation strategy ( i BOPs) and an in-StageTip technique. This novel and straightforward approach allows for the rapid solubilization and processing of a variety of IMPs from human samples to support highly sensitive mass spectrometry analysis. We also demonstrated that the performance of this technology surpasses that of conventional methods such as filter-aided sample preparation methods, FASP and i -FASP. The convenience and availability of pTRUST technology using the IL system have great potential for proteomic identification and characterization of novel drug targets and disease biology in research and clinical settings.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0003269723003147; http://dx.doi.org/10.1016/j.ab.2023.115349; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85174439954&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/37852348; https://linkinghub.elsevier.com/retrieve/pii/S0003269723003147; https://dx.doi.org/10.1016/j.ab.2023.115349
Elsevier BV
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