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Construction of a competitive electrochemical immunosensor based on sacrifice of Prussian blue and its ultrasensitive detection of alpha-fetoprotein

Analytica Chimica Acta, ISSN: 0003-2670, Vol: 1257, Page: 341143
2023
  • 9
    Citations
  • 0
    Usage
  • 5
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    9
    • Citation Indexes
      9
  • Captures
    5
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

Findings from Shihezi University Has Provided New Data on Fetal Proteins (Construction of a Competitive Electrochemical Immunosensor Based On Sacrifice of Prussian Blue and Its Ultrasensitive Detection of Alpha-fetoprotein)

2023 MAY 26 (NewsRx) -- By a News Reporter-Staff News Editor at Chemicals & Chemistry Daily Daily -- Data detailed on Proteins - Fetal Proteins

Article Description

Effective signal amplification is a prerequisite for ultrasensitive detection by electrochemical immunosensors. For quantitative and ultrasensitive detection of alpha-fetoprotein (AFP), we designed a competitive electrochemical immunosensor and transferred the immunoreactivity from the electrode surface to the cuvette. AFP antigen was captured using AFP primary antibody (Ab 1 ) immobilized on magnetic nanobeads (MBs), and ZIF-8 nanomaterials attached to secondary antibody (Ab 2 ) were used as probes. MBs helped retain the sandwich structure in the test tube through incubation and washing steps. Then, an appropriately fixed excess of sodium ethylenediaminetetraacetic acid (EDTA) solution was added to the cuvettes, resulting in etching of Zn ions from ZIF-8 and formation of Zn-EDTA complexes. After magnetic separation, a certain amount of supernatant is added dropwise to the Prussian blue (PB)-modified electrode (GCE), and Fe ions (from PB) complex with the remaining EDTA in the supernatant, thus reducing the signal response value of PB. The higher the AFP concentration, the lower the amount of free EDTA in the supernatant, the less the destruction of PB, and therefore the higher the current. Under optimal conditions, the immunosensor achieved ultra-sensitive detection of AFP in the range of 10 −4 ng/mL-100 ng/mL with a limit of detection (LOD) as low as 0.032 pg/mL (S/N = 3). The excellent performance provides an important tool for the early screening and detection of AFP.

Bibliographic Details

Wang, Min; Jiang, Mingzhe; Li, Pengli; Yuan, Mengting; Zhao, Chulei; Lai, Wenjing; Li, Jiajia; Hong, Chenglin; Qi, Yu

Elsevier BV

Chemistry; Environmental Science; Biochemistry, Genetics and Molecular Biology

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