Functional characterization of carotenogenic genes provides implications into carotenoid biosynthesis and engineering in the marine alga Nannochloropsis oceanica
Algal Research, ISSN: 2211-9264, Vol: 67, Page: 102853
2022
- 16Citations
- 23Captures
- 1Mentions
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Most Recent News
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Article Description
Nannochloropsis oceanica represents a promising sunlight-driven eukaryotic cell factory for lipids and value-added products. Despite the well-studied lipid metabolism, carotenoid biosynthesis in this alga remains less understood. Here we first identified the putative carotenogenic genes via bioinformatics analysis and reconstructed the pathways for carotenoid biosynthesis in N. oceanica. Two key carotenogenic genes, phytoene desaturase (NoPDS) and lycopene β -cyclase (NoLCYB), were then selected for further investigation. Both were functionally validated in the engineered Escherichia coli strains and demonstrated to reside in the chloroplast of N. oceanica. Interestingly, whilst NoPDS overexpression showed little effect on carotenoid profiles, NoLCYB overexpression promoted the level of carotenoids particularly β -carotene, suggesting that the step mediated by NoLCYB rather than NoPDS is rate-limiting for carotenoid biosynthesis in N. oceanica. Moreover, a NoPDS variant generated by site-directed mutagenesis was developed as a dominant selectable marker alternative to the antibiotic resistance genes of bacterial origin for N. oceanica transformation. Our results not only help understand carotenoid biosynthesis in N. oceanica, but also expand selectable marker of endogenous origin and provide implications into future genetic engineering of this alga for carotenoid manipulation.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S2211926422002247; http://dx.doi.org/10.1016/j.algal.2022.102853; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85138101148&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S2211926422002247; https://dx.doi.org/10.1016/j.algal.2022.102853
Elsevier BV
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