Production of superoxide anion and hydrogen peroxide by capacitating buffalo ( Bubalus bubalis ) spermatozoa
Animal Reproduction Science, ISSN: 0378-4320, Vol: 103, Issue: 3, Page: 260-270
2008
- 13Citations
- 17Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations13
- Citation Indexes13
- 13
- CrossRef10
- Captures17
- Readers17
- 17
Article Description
In the present study attempts were made to detect and quantify the generation of superoxide anion (O 2 − ) and hydrogen peroxide (H 2 O 2 ) by capacitating buffalo spermatozoa. Ejaculated buffalo spermatozoa were suspended in sp-TALP medium at 50 × 10 6 mL −1 and incubated at 38.5 °C with 5% CO 2 in air in the absence or presence of heparin (a capacitation inducer) or reduced nicotinamide adenine dinucleotide phosphate (NADPH) or diphenyleneiodonium (DPI, a flavoprotein inhibitor) for 6 h. Production rate of O 2 − and H 2 O 2 by spermatozoa at different hours of capacitation were measured by cytochrome c reduction and phenol red oxidation assays, respectively. Spermatozoa generated both O 2 − and H 2 O 2 spontaneously and following stimulation with heparin and a significant increase of O 2 − production was observed in the presence of NADPH. However, DPI inhibited this NADPH-induced O 2 − production and suggested for existence of putative NADPH-oxidase that constitute a specific O 2 − generating systems in buffalo spermatozoa. Results of our study indicated that buffalo spermatozoa generate O 2 − and H 2 O 2 and production of these free radicals is induced during capacitation.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0378432007000024; http://dx.doi.org/10.1016/j.anireprosci.2007.01.001; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=36549058341&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/17270367; https://linkinghub.elsevier.com/retrieve/pii/S0378432007000024; https://dx.doi.org/10.1016/j.anireprosci.2007.01.001
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know