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Investigating the effect of VEGF glycosylation on glycosaminoglycan binding and protein unfolding

Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 340, Issue: 3, Page: 836-839
2006
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Article Description

VEGF 165 binding to endothelial cells is potentiated by glycosaminoglycans (GAGs). Here, we have investigated the impact of VEGF 165 N-glycosylation on GAG binding. Although glycosylated VEGF 165 bound to heparin with only slightly higher affinity than non-glycosylated VEGF 165, the natural ligand heparan sulfate induced a conformational change only in the glycosylated protein. Unfolding studies of the VEGF proteins indicated a stabilising effect of heparin on the growth factor structure.

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