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Effects of ethanol on cytokine generation and NFκB activity in human lung epithelial cell

Biochemical Pharmacology, ISSN: 0006-2952, Vol: 70, Issue: 4, Page: 545-551
2005
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Article Description

Alcohol abuse is associated with enhanced risk for pulmonary infections, but the mechanisms remain obscure. We assessed whether ethanol reduced generation of cytokines from a human lung epithelial cell line (A549) in vitro and if effects on the NFκB transcription factor were involved. Exposure of A549 to ethanol (0.1–1%) dose-dependently inhibited (by 15–49%) the release of G-CSF and IL-8, but not of M-CSF, triggered by IL1β or TNFα. Ethanol also inhibited by 49% the IL-1β stimulated translocation of the p65 subunit of NFκB from the cytoplasm into the nucleus. Using a κB binding and luciferase coupled construct, transfected into A549 cells, we found that 1% ethanol specifically reduced IL-1β and TNFα induced luciferase activity with 34 and 40%, respectively. Thus, in vitro exposure of lung epithelial cells to ethanol reduced the generation of cytokines, as well as translocation and gene activation by NFκB.

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