Recent developments in microalgal genome editing for enhancing lipid accumulation and biofuel recovery
Biomass and Bioenergy, ISSN: 0961-9534, Vol: 150, Page: 106093
2021
- 18Citations
- 29Captures
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Review Description
Microalgae based biofuel has been extensively researched with huge investment over more than a past decade. However, integration of lipid accumulation and recovery needs to be optimized for economical production of biofuel. Microalgae are promising feedstocks for carbon-neutral biofuels and their commercialization depends on strain improvement by targeted inhibition of desired gene to increase lipid content. The current review describes the triacylglycerol, phospholipid, fatty acid, starch biosynthetic pathway and target gene for metabolic engineering. Further, it focuses on genome editing techniques viz., Clustered Regularly Interspaced Short Palindromic Repeats, RNA Interference, Transcription Activator-Like Effector Nucleases, Mutation and chemical inhibitor to enhance triacylglycerol content together with cultivation and biomass harvesting for fuel precursor extraction. It also highlights the latest development, challenges for integrating single cell oil accumulation and extraction methods like, biomass pretreatment, solvent based cell lysis and solvent free green technologies for fuel conversion. Future efforts on microalgal extracellular matrix, organelle membrane biology, continuous production of oil and non-destructive oil recovery from growth media need to be addressed for large scale commercial production of biofuel.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0961953421001306; http://dx.doi.org/10.1016/j.biombioe.2021.106093; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85105338424&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0961953421001306; https://dx.doi.org/10.1016/j.biombioe.2021.106093
Elsevier BV
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