Metabolic engineering of Bacillus subtilis for the efficient biosynthesis of uniform hyaluronic acid with controlled molecular weights
Bioresource Technology, ISSN: 0960-8524, Vol: 132, Page: 427-431
2013
- 93Citations
- 138Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations93
- Citation Indexes93
- 93
- CrossRef66
- Captures138
- Readers138
- 138
Article Description
Bacillus subtilis was engineered into an efficient hyaluronic acid (HA) producer by introducing two inducible artificial operons carrying HA synthase gene from Pasteurella multocida and precursor genes encoding enzymes involved in synthesis of the sugar precursors. A two-stage induction strategy was established for metabolic engineering of recombinant B. subtilis to efficiently produce uniform HA with controlled molecular weights. Strain TPG223 produced larger HA molecules (yield = 6.8 g/L; molecular weight = 4.5 MDa) than strain PG6181 (yield = 2.4 g/L; molecular weight = 13 KDa), indicating that the enzymes involved in the synthesis of UDP-glucuronic acid are essential for HA biosynthesis. Strain TPG223 was able to synthesize HA molecules ranging in molecular weight from 8 KDa to 5.4 MDa indicating that size control is achievable in vivo through appropriate tools. The work reported here not only advanced mechanisms research of size control in vivo, but also could be an attractive alternative for commercial preparation of uniform size-defined HA.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0960852412020081; http://dx.doi.org/10.1016/j.biortech.2012.12.150; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84875251441&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/23433979; https://linkinghub.elsevier.com/retrieve/pii/S0960852412020081; https://dx.doi.org/10.1016/j.biortech.2012.12.150
Elsevier BV
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