Mechanisms of nitrogen transformation driven by functional microbes during thermophilic fermentation in an ex situ fermentation system

In this study, we explored the pathways and mechanisms of nitrogen (N) transformation driven by functional microbes carrying key genes in an ex situ fermentation system (EFS). Temperature and N content were found to be the most important factors driving variation in bacterial and fungal communities, respectively; Bacillus became the most abundant bacteria and Batrachochytrium became the most abundant fungi. Co-occurrence network analysis showed that some bacteria including Halomonas, Truepera, and Gemmatimonas species carry genes that promote mineralization, nitrification, dissimilatory/assimilatory nitrate reduction, denitrification, anammox reactions, and N fixation. The maximum rate of total mineralization reached 136.60 μg N g −1 d −1. Functional microbes promoted various N conversion processes at different rates in the EFS, with levels increasing by at least 0.23 μg N g −1 d −1. These results provide a theoretical basis for feasible optimization measures to address N loss during fermentation.