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Impact of carbon/nitrogen ratio on sequencing batch biofilm reactors initiated with different seed sludges for treating actual mariculture effluents

Bioresource Technology, ISSN: 0960-8524, Vol: 417, Page: 131838
2025
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The impact of carbon/nitrogen (C/N) ratio on sequencing batch biofilm reactor (SBBR) initiated with different seed sludges for treating actual mariculture effluent was explored. Increasing the C/N ratio significantly enhanced the nitrogen removal efficiency, achieving average removal efficiency of 95% for ammonia nitrogen and 73% for total nitrogen at ratio of 30, while the impact of seed sludge was minimal. High C/N ratio promoted the secretion of tightly bound extracellular polymeric substances (TB-EPS), which showed significant correlation with nitrogen removal. Interactions between bacteria and archaea were enhanced and conditionally rare or abundant taxa were the keystone taxa. High C/N ratio inhibited the relative abundance of ammonia-oxidizing archaea ( Candidatus_Nitrosopumilus ) and bacteria ( Nitrosomonas ), but promoted the heterotrophic nitrification-aerobic denitrification bacteria ( Halomonas ). The expression of nitrogen removal functional genes significantly correlated with functional genera. This study emphasized the crucial role of high C/N ratios in biological nitrogen removal from actual mariculture effluent.

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