A Slowed Classical Pathway Rather Than Kiss-and-Run Mediates Endocytosis at Synapses Lacking Synaptojanin and Endophilin
Cell, ISSN: 0092-8674, Vol: 123, Issue: 3, Page: 521-533
2005
- 154Citations
- 148Captures
- 1Mentions
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Metrics Details
- Citations154
- Citation Indexes154
- 154
- CrossRef135
- Captures148
- Readers148
- 148
- Mentions1
- References1
- Wikipedia1
Article Description
The extent to which a “kiss-and-run” mode of endocytosis contributes to synaptic-vesicle recycling remains controversial. The only genetic evidence for kiss-and-run at the synapse comes from mutations in the genes encoding synaptojanin and endophilin, proteins that together function to uncoat vesicles in classical clathrin-mediated endocytosis. Here we have characterized the endocytosis that persists in null alleles of Drosophila synaptojanin and endophilin. In response to high-frequency stimulation, the synaptic-vesicle pool can be reversibly depleted in these mutants. Recovery from this depletion is slow and indicates the persistence of an impaired form of classical endocytosis. Steady-state exocytosis rates reveal that endocytosis saturates in mutant neuromuscular terminals at ∼80 vesicles/s, 10%–20% of the wild-type rate. Analyses of quantal size, FM1-43 loading, and dynamin function further demonstrate that, even in the absence of synaptojanin or endophilin, vesicles undergo full fusion and re-formation. Therefore, no genetic evidence remains to indicate that synaptic vesicles undergo kiss-and-run.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0092867405009803; http://dx.doi.org/10.1016/j.cell.2005.09.026; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=27544489397&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/16269341; https://linkinghub.elsevier.com/retrieve/pii/S0092867405009803
Elsevier BV
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