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Natural infection by Zika virus but not DNA vaccination consistently elicits antibodies that compete with two potently neutralising monoclonal antibodies targeting distinct epitopes

eBioMedicine, ISSN: 2352-3964, Vol: 98, Page: 104875
2023
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Metric Options:   Counts1 Year3 Year

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  • Captures
    21
  • Mentions
    1
    • News Mentions
      1
      • 1
  • Social Media
    20
    • Shares, Likes & Comments
      20
      • Facebook
        20

Most Recent News

Researchers from Emory University Report Findings in Zika Virus (Natural Infection By Zika Virus but Not Dna Vaccination Consistently Elicits Antibodies That Compete With Two Potently Neutralising Monoclonal Antibodies Targeting Distinct ...)

2024 JAN 18 (NewsRx) -- By a News Reporter-Staff News Editor at Vaccine Daily -- Investigators publish new report on Mosquito-Borne Diseases - Zika Virus.

Article Description

Autochthonous transmission of Zika virus (ZIKV) has been reported in 87 countries since 2015. Although most infections are mild, there is risk of Guillain-Barré syndrome and adverse pregnancy outcomes. Vaccines are urgently needed to prevent Zika, but sufficient understanding of humoral responses and tools to assess ZIKV-specific immunity are lacking. We developed a blockade-of-binding (BOB) ELISA using A9E and G9E, two strongly neutralising ZIKV-specific monoclonal antibodies, which do not react with dengue virus. Receiver operating characteristic curve analysis assessed A9E and G9E BOB serodiagnostic performance. BOB was then applied to samples from a surveillance cohort in Risaralda, Colombia, and phase 1 ZIKV vaccine trial samples, comparing results against traditional serologic tests. In the validation sample set (n = 120), A9E BOB has a sensitivity of 93.5% (95% CI: 79.3, 98.9) and specificity 97.8 (95% CI: 92.2, 99.6). G9E BOB had a sensitivity of 100% (95% CI: 89.0, 100.0) and specificity 100% (95% CI: 95.9, 100). Serum from natural infections consistently tested positive in these assays for up to one year, and reactivity tracks well with ZIKV infection status among sera from endemic areas with complicated flavivirus exposures. Interestingly, a leading ZIKV vaccine candidate elicited minimal BOB reactivity despite generating neutralising antibody responses. In conclusion, A9E and G9E BOB assays are sensitive and specific assays for detecting antibodies elicited by recent or remote ZIKV infections. Given the additional ability of these BOB assays to detect immune responses that target different epitopes, further development of these assays is well justified for applications including flavivirus surveillance, translational vaccinology research and as potential serologic correlates of protective immunity against Zika. R21 AI129532 (PI: S. Becker-Dreps), CDC BAA 2017-N-18041 (PI: A. M. de Silva), Thrasher Fund (PI: M. H. Collins), K22 AI137306 (PI: M. H. Collins).

Bibliographic Details

Smith, Teresa C; Espinoza, Daniel O; Zhu, Yerun; Cardona-Ospina, Jaime A; Bowman, Natalie M; Becker-Dreps, Sylvia; Rouphael, Nadine; Rodriguez-Morales, Alfonso J; Bucardo, Filemon; Edupuganti, Srilatha; Premkumar, Lakshmanane; Mulligan, Mark J; de Silva, Aravinda M; Collins, Matthew H

Elsevier BV

Biochemistry, Genetics and Molecular Biology

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