Multiple antibody conjugation to PEG grafted copolymer improves sensitivity of immunoassays
European Polymer Journal, ISSN: 0014-3057, Vol: 216, Page: 113263
2024
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Most Recent News
New Immunoglobulins Findings from University of Claude Bernard Lyon 1 Discussed (Multiple Antibody Conjugation To Peg Grafted Copolymer Improves Sensitivity of Immunoassays)
2024 AUG 14 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Daily -- Data detailed on Immunology - Immunoglobulins have been
Article Description
A polyethylene glycol (PEG) grafted copolymer based on a N-vinyl pyrrolidone (NVP) backbone was designed for conjugating antibodies (Abs) at PEG chain ends, leading to multivalent (“multi-antibody”) bioconjugate for use as a detection system in immunoassays. For this purpose, a poly(N-vinyl pyrrolidone-co-N-acryloxysuccinimide) (P(NVP-co-NAS)) copolymer was prepared by nitroxide mediated polymerization (NMP) and the N-succinimidyl activated esters of the NAS units were used for grafting heterobifunctional amine-PEG-maleimide by amidation. The PEG maleimide end-groups were further conjugated to Abs through thiol-maleimide addition. Enzyme-linked immunosorbent assay (ELISA) performed with the obtained bioconjugate in the detection phase showed significant improvement in antigen detection as compared to standard protocol with free Ab. These results point out the relevance of this straightforward approach to improve antibody accessibility and reduce background noise (thanks to anti-fouling PEG) for enhanced sensitivity in the field of immunoassays.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S001430572400524X; http://dx.doi.org/10.1016/j.eurpolymj.2024.113263; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85197374472&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S001430572400524X; https://dx.doi.org/10.1016/j.eurpolymj.2024.113263
Elsevier BV
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