A multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed fish products
Food Chemistry, ISSN: 0308-8146, Vol: 122, Issue: 1, Page: 319-326
2010
- 37Citations
- 40Captures
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Article Description
In this study, we have developed a novel multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed food. The method consists of two novel Scomber japonicus - (104 bp) and Scomber australasicus -specific (143 bp) amplicons, respectively, corresponding to the mitochondrial control region. It also includes the previously described Scomber colias -specific product (159 bp) corresponding to the 5S ribosomal DNA, the Scomber scombrus -specific fragment (123 bp) from the mitochondrial NADH dehydrogenase subunit 5, and finally a positive amplification control corresponding to the small 12S rRNA subunit (188 bp). The system was assayed in fresh samples as well as in a total of 40 commercial samples including 28 different canned products and 12 unprocessed fresh fillets. A positive identification was observed in all cases according to their commercial labelling. Overall, this methodology reveals as a potential molecular tool for direct application in the authentication of Scomber mackerels in the seafood industry.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0308814610002207; http://dx.doi.org/10.1016/j.foodchem.2010.02.036; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77950691051&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0308814610002207; https://api.elsevier.com/content/article/PII:S0308814610002207?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:S0308814610002207?httpAccept=text/plain; https://dx.doi.org/10.1016/j.foodchem.2010.02.036
Elsevier BV
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