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A multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed fish products

Food Chemistry, ISSN: 0308-8146, Vol: 122, Issue: 1, Page: 319-326
2010
  • 37
    Citations
  • 0
    Usage
  • 40
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    37
    • Citation Indexes
      37
  • Captures
    40

Article Description

In this study, we have developed a novel multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed food. The method consists of two novel Scomber japonicus - (104 bp) and Scomber australasicus -specific (143 bp) amplicons, respectively, corresponding to the mitochondrial control region. It also includes the previously described Scomber colias -specific product (159 bp) corresponding to the 5S ribosomal DNA, the Scomber scombrus -specific fragment (123 bp) from the mitochondrial NADH dehydrogenase subunit 5, and finally a positive amplification control corresponding to the small 12S rRNA subunit (188 bp). The system was assayed in fresh samples as well as in a total of 40 commercial samples including 28 different canned products and 12 unprocessed fresh fillets. A positive identification was observed in all cases according to their commercial labelling. Overall, this methodology reveals as a potential molecular tool for direct application in the authentication of Scomber mackerels in the seafood industry.

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