Gene expression of certain heat shock proteins and antioxidant enzymes in microwave exposed rats

Cellular interaction between microwave radiations 2.45GHz frequency and cellular function is very important in explaining the role played by the cellular signaling pathway in keeping cell function within normal condition. The aim of this study is to examine the gene expression of certain heat shock proteins and antioxidant enzymes in microwave exposed rats. Thirty female rats at the age of three months were divided into two groups: control and exposed groups, whole body exposure for 2 h/day up to 60 days at SAR value 0.1434 W/Kg were employed. On the day 60 of experiment, liver samples were collected from scarified animals for RNA isolation and heat shock protein and antioxidant enzymes genes expression by RT-qPCR. The results showed up and down regulations of the studied genes in exposed rats compared to control rats. Also, there is a strong linear relationship between antioxidant enzymes and heat shock protein expression; there is a strong linear relationship between (catalase and GST) genes and GSH-Px1gene expression. The study concludes that the 2.45GHz microwave radiation induced alteration in certain heat stress proteins and antioxidant enzymes gene expression at the cellular level as a consequence of cellular toxicity resulted from oxidative stress induction.