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Heterogeneous response of Geobacillus stearothermophilus biofilms to calcium

International Dairy Journal, ISSN: 0958-6946, Vol: 116, Page: 104961
2021
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Article Description

Geobacillus stearothermophilus contamination of milk powder products originates from bacteria released from biofilms in dairy manufacturing plants. Calcium, a major component of milk minerals, was previously found to affect G. stearothermophilus biofilm growth on surfaces exposed to milk formulations. This study endeavoured to reproduce the effect of calcium on biofilm formation of several G. stearothermophilus dairy isolates and one reference strain in modified TSB media and gain more insights into the role of calcium in biofilm formation of G. stearothermophilus. Calcium presence increased biofilm cell numbers of strain A1, but reduced cell numbers of the reference strain and showed no significant effect on strain P3. The quantity of extracellular polymeric substances (EPS), in particular extracellular protein, varied between strains. Unlike the consistent effect of calcium in milk formulations found previously, current findings suggest that strain to strain differences exists in biofilm formation of G. stearothermophilus in the presence of calcium.

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