A differential scanning calorimetric study of the influence of copper and dodecyl trimethyl ammonium bromide on the stability of bovine α-lactalbumin
International Journal of Biological Macromolecules, ISSN: 0141-8130, Vol: 36, Issue: 3, Page: 169-175
2005
- 11Citations
- 10Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Bovine α-lactalbumin (α-LA) has been studied by differential scanning calorimetry (DSC), fluorescence spectroscopy and viscometry with various concentrations of Cu 2+ and DTAB to elucidate the effect of these ligands on its thermal properties. The DSC profile of dialyzed form of α-lactalbumin (m-α-LA) contrary to the undialyzed form (holo-form, h-α-LA) shows two temperature induced heat absorption peaks. The m-α-LA is not a new form of α-LA. It contains mixture of the apo (a-α-LA) and holo (h-α-LA) forms of α-LA at low and high temperatures, respectively. Therefore, these two states of α-LA (apo and holo) are equilibrating with together after dialyze experiment. The Cu 2+ as a metal ion and DTAB as a non metal ion alter the two heat-absorption peaks, in such a manner that, the addition of Cu 2+ to the m-α-LA increases partial molar heat capacity and enthalpy change values of the h-α-LA form at high temperature because the molecular population of the a-α-LA form changes into the h-like-α-LA. On the contrary, the interaction between the DTAB and the m-α-LA increases these thermodynamic values for the a-α-LA at low temperature. However, DTAB bound to m-α-LA prevents from Ca 2+ binding to protein, because there are positive charges repulsion between them. The high temperature peak occurs at the same temperature as the unfolding of the h-α-LA, while the low temperature peak lies within the temperature range associated with the unfolding of the a-α-LA. The R s values of m-α-LA, h-α-LA and a-α-LA forms confirmed the folding and unfolding of the m-α-LA during the addition of Cu 2+ and DTAB at different concentration, respectively.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0141813005000863; http://dx.doi.org/10.1016/j.ijbiomac.2005.05.005; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=23044497761&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/16019061; https://linkinghub.elsevier.com/retrieve/pii/S0141813005000863; https://dx.doi.org/10.1016/j.ijbiomac.2005.05.005
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know