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Ultrasensitive and rapid detection of organophosphates using a dual-signal naked-eye hydrogel sensor based on acetylcholinesterase inhibition

International Journal of Biological Macromolecules, ISSN: 0141-8130, Vol: 283, Issue: Pt 2, Page: 137778
2024
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Article Description

Accurate determination of pesticide residues is crucial for food safety. A self-calibration method was developed for dual-signal “naked-eye” detection of organophosphorus pesticides (OPs) using bifunctional gold nanozymes (AuNEs). OPs inhibit the cascade reaction of acetylcholinesterase/choline oxidase (AChE/CHO) to reduce hydrogen peroxide (H 2 O 2 ) production, which affects the AuNE-catalyzed color reaction and quenches the fluorescence of AuNEs with the assistance of Fe 2+. The multi-enzyme cascade system can be integrated into hydrogel sheets for smartphone-assisted digital, quantitative, and onsite detection of OPs in real food samples by analyzing the linear changes in colorimetric and fluorometric signals, which closely match liquid chromatography-mass spectrometry (LC-MS) results. This method displays a lowest ‘naked-eye’ detection concentration of 7 ppb, a wide linear range (0–2000 ng/mL), and particularly the advantage of anti-interference via self-calibration when one of the signals is abnormal.

Bibliographic Details

Wang, Jiachun; Huang, Yibing; Kuai, Ziyu; Zhang, Yanping; Shen, Qi; Tian, Pujing; Nong, Weihai; Jiang, Wantong; He, Yuting; Ran, Nana; Yin, Yinuo; Li, Tiezhu; Luo, Quan

Elsevier BV

Biochemistry, Genetics and Molecular Biology

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