Insights into the relationship between the acetylation of Dendrobium officinale polysaccharides and the ability to promote sIgA secretion

The acetyl group is a significant reactive component of Dendrobium officinale polysaccharide (DOP). In this study, we prepared DOPs with different degrees of acetyl substitution and investigated how the acetyl group, a naturally occurring characteristic of DOP, influences the immunomodulatory activity and the production of secretory IgA (sIgA) in the small intestine. Physical property measurements revealed significant changes in surface morphology and solubility of DOP caused by the addition or removal of acetyl groups. In vivo studies have demonstrated that DOP can mitigate Cyclophosphamide-induced immunosuppression by enhancing the immune organ index, promoting immunoglobulin secretion, and increasing the population of immune cells. Additionally, DOP can enhance sIgA production through multiple pathways, including enhanced IgA + B cell class switch recombination, gut homing of IgA + plasma cells, and upregulation of factors involved in sIgA composition and secretion. Correlation analysis revealed strong, piecewise-specific correlations between DOP acetylation and sIgA production at varying intervals of acetyl substitution. Based on this, we propose a theoretical framework in which the acetylation of DOP and the secretion of small intestinal sIgA demonstrate a “piecewise correlation”. This framework illustrates the influence of DOP acetylation on immunomodulatory activity and provides a theoretical basis for enhancing the added value of Dendrobium officinale resources.