Evaluation of bioaerosol sampling techniques for Legionella pneumophila coupled with culture assay and quantitative PCR
Journal of Aerosol Science, ISSN: 0021-8502, Vol: 41, Issue: 12, Page: 1055-1065
2010
- 22Citations
- 27Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Efficient bioaerosol sampling methods are required for characterization of exposure risk to airborne pathogenic Legionella pneumophila, which may cause severe pneumonia and death in humans. By using culture assay and quantitative PCR, the effects of sampler type, sampling time (1–60 min), collection fluid (deionized water (DW) and Tween mixture), and DW replenishment during sampling on the recovery of culturable and total L. pneumophila were assessed. Escherichia coli was also tested as a comparison. The collection efficiency was consistently greater for culturable L. pneumophila than E. coli by a factor of 2.7–12.2 ( P =0.005). Besides, total and culturable bioaerosols were both recovered less in microbiological air sampler (MAS-100) by 1–2 orders of magnitude than in Andersen one-stage sampler (Andersen 1-STG), all-glass impinger (AGI-30), and BioSampler regardless of sampling time, bacterial species ( L. pneumophila and E. coli ), and collection principle (agar impaction and liquid impingement) ( P <0.05). BioSampler was more efficient than AGI-30 to capture total L. pneumophila ( P =0.003), whereas a comparable bioefficiency was found for culturable cells ( P =0.74). DW obtained more culturable L. pneumophila than Tween mixture by a factor of 4.1–12.5 in BioSampler and 2.2–6.8 in AGI-30 during 3–60 min sampling ( P <0.0001). Increasing sampling time deteriorated the accuracy of L. pneumophila quantification, particularly for culturable cells ( P <0.0001). However, DW replenishment every 15 min during sampling significantly improved the recovery of culturable L. pneumophila in both AGI-30 and BioSampler by a factor of 3.3 and 7.1, respectively ( P =0.001). Considering a longer sampling is preferred to quantifying airborne L. pneumophila usually at a low level of concentration, this study recommends to collecting this pathogen by BioSampler with periodical refilling of DW.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021850210001904; http://dx.doi.org/10.1016/j.jaerosci.2010.09.002; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78049500587&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0021850210001904; https://api.elsevier.com/content/article/PII:S0021850210001904?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:S0021850210001904?httpAccept=text/plain; https://dx.doi.org/10.1016/j.jaerosci.2010.09.002
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know