Metabolic engineering of Pichia pastoris for production of hyaluronic acid with high molecular weight
Journal of Biotechnology, ISSN: 0168-1656, Vol: 185, Page: 28-36
2014
- 91Citations
- 169Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations91
- Citation Indexes91
- 91
- CrossRef76
- Captures169
- Readers169
- 169
Article Description
The high molecular weight (>1 MDa) of hyaluronic acid (HA) is important for its biological functions. The reported limiting factors for the production of HA with high molecular weight (MW) by microbial fermentation are the insufficient HA precursor pool and cell growth inhibition. To overcome these issues, the Xenopus laevis xhasA2 and xhasB genes encoding hyaluronan synthase 2 ( xhasA2 ) and UDP-glucose dehydrogenase ( xhasB ), were expressed in Pichia pastoris widely used for production of heterologous proteins. In this study, expression vectors containing various combination cassettes of HA pathway genes including xhasA2 and xhasB from X. laevis as well as UDP-glucose pyrophosphorylase ( hasC ), UDP-N-acetylglucosamine pyrophosphorylase ( hasD ) and phosphoglucose isomerase ( hasE ) from P. pastoris were constructed and tested. First, HA pathway genes were overexpressed using pAO815 and pGAPZB vectors, resulting in the production of 1.2 MDa HA polymers. Second, in order to decrease hyaluronan synthase expression a strong AOX1 promoter in the xhasA2 gene was replaced by a weak AOX2 promoter which increased the mean MW of HA to 2.1 MDa. Finally, the MW of HA polymer was further increased to 2.5 MDa by low-temperature cultivation (26 °C) which reduced cell growth inhibition. The yield of HA production by the P. pastoris recombinant strains in 1 L of fermentation culture was 0.8–1.7 g/L.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0168165614002648; http://dx.doi.org/10.1016/j.jbiotec.2014.05.018; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84903474498&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/24892811; https://linkinghub.elsevier.com/retrieve/pii/S0168165614002648; https://dx.doi.org/10.1016/j.jbiotec.2014.05.018
Elsevier BV
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