Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains
Journal of Clinical Virology, ISSN: 1386-6532, Vol: 48, Issue: 3, Page: 187-192
2010
- 55Citations
- 52Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations55
- Citation Indexes49
- 49
- CrossRef41
- Policy Citations6
- Policy Citation6
- Captures52
- Readers52
- 52
Article Description
Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations. To develop a real-time assay for YFV able to detect African and South American strains. Three short probe (14–18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested. A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes. The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1386653210001927; http://dx.doi.org/10.1016/j.jcv.2010.04.013; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77953120432&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/20556888; https://linkinghub.elsevier.com/retrieve/pii/S1386653210001927; https://dx.doi.org/10.1016/j.jcv.2010.04.013
Elsevier BV
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