Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption
Journal of Hazardous Materials, ISSN: 0304-3894, Vol: 461, Page: 132656
2024
- 5Citations
- 8Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Article Description
Conventionally, the measurement of metal ion adsorption capacity in biosorbent relies on expensive and time-consuming ICP-OES technique. Herein, a semi-quantitative method to measure Pd(II) adsorption capacity of single cells has been presented by analyzing side scatter (SSC) intensity in flow cytometry. Within the sensitive range and applicable conditions, excellent linearity correlation (R 2 ranges from 0.89 to 0.96) between the amount of Pd(II) absorbed on yeast and the fold increase in SSC intensity has been observed. Using this method, six strains with high Pd adsorption capacities have sorted from a yeast library with metal-binding peptides displayed (up to 10 7 strains) based on SSC signal intensity. The optimal peptide (EF1) displayed on yeast and E. coli surface demonstrated Pd adsorption improvements of ∼32% and ∼200%, respectively. In summary, our study proposes an alternative high-throughput method for analyzing the Pd(II) adsorption capacity of individual yeast cells, enabling the screening of specific peptides/proteins with high Pd(II) affinity from extensive libraries.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0304389423019398; http://dx.doi.org/10.1016/j.jhazmat.2023.132656; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85173167308&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/37793255; https://linkinghub.elsevier.com/retrieve/pii/S0304389423019398; https://dx.doi.org/10.1016/j.jhazmat.2023.132656
Elsevier BV
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