Effect of Colchicum ritchii derived nanoparticles on apple ring rot disease without changing the taste through the prevented metabolites changes and increased sialic acid synthesis

In this current study, we were planned to produce Colchicum ritchii flower extract associated calcium chloride nanoparticle (CRNPs) in a simple organic method and evaluate their apple fruit pathogenic resistance as well as their shelf-life extension properties during storage. This CRNPs was confirmed by analysis of various established physical and chemical methods. Moreover, the apple fruit pathogenic resistance as well as their shelf-life extension properties during storage were evaluated by standardizing methods. Our results showed that the CRNPs were contrived and proven by physical and chemical evaluations. Moreover, the biological observation of CRNPs indicates that they have good pathogenic resistance properties against the Botryosphaeria dothidea without changing the taste through the prevented metabolites changes and increased sialic acid synthesis. The effective dose of pathogenic resistance in CRNPs was 40 µg/mL. The levels of defensive enzymes such as chitinase (CHI), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), and β-1,3-glucanase (GLU) significantly increased in CRNPs treated fruits. The increasing antioxidant enzymes activities including superoxide dismutase (SOD) and catalase (CAT) were shown in CRNPs treated apple fruit. The CRNPs-treated apple fruit was found to increase in salicylic acid (SA) and SA glucoside (SAG) levels, owing to increased SA synthesis. Moreover, shelf-life extension studies of fresh cut apples were conducted by immersing them in 20 and 40 µg/mL CRNPs as well as a standard solution of 2% calcium chloride for 5 mins. The standard solution of calcium chloride as well the CRNPs were proved as a good preservative and they were preserved the apple fruits shelf-life up to 25 days. In this study, we have concluded that the CRNPs were produced by fusion with Colchicum ritchii flower extract and calcium chloride in a superlative natural method. Our results recommended that the CRNPs is acted as a best source for apple ring rot resistance without changing the taste through the prevented metabolites changes and increased sialic acid synthesis as well as the antioxidant properties. In addition, the CRNPs is superlative source for apple shelf-life extension up to 25 days without any toxicology nature.