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Down-regulated reactive oxygen species by HSP90 in 3HK-induced SKN-SH cell death

Journal of Thermal Biology, ISSN: 0306-4565, Vol: 30, Issue: 1, Page: 43-49
2005
  • 5
    Citations
  • 0
    Usage
  • 17
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    5
    • Citation Indexes
      5
  • Captures
    17

Article Description

In this present study, we show that 3HK induced reactive oxygen species (ROS) accumulation and after caspase activation lead to apoptotic cell death. Pretreatment with N -acetylcysteine (NAC), an effective antioxidant, significantly attenuated 3HK-induced apoptosis by way of a reduction of ROS accumulation and caspase activity. SKN-SN cells were protected from 3HK-induced cytotoxicity by heat shock protein (HSP). HSP effectively attenuated 3HK-mediated ROS accumulation and apoptosis. In addition, the protective effect of HSP90 was abolished by pretreatment with HSP90 anti-sense oligonucleotides, but not when pretreated with anti-senses for other HSPs. These results suggest that HSP90 protects SKN-SH cells from 3HK-induced cytotoxicity by reducing ROS levels and caspase activity.

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