Recombinase polymerase amplification assay for sensitive and rapid detection of southern rice black-streaked dwarf virus in plants
Journal of Virological Methods, ISSN: 0166-0934, Vol: 301, Page: 114467
2022
- 3Citations
- 9Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations3
- Citation Indexes3
- CrossRef1
- Captures9
- Readers9
Article Description
Southern rice black-streaked dwarf virus (SRBSDV) naturally infects rice and maize plants through white-backed planthopper ( Sogatella furcifera ) causing significant crop losses in China and Vietnam. Thus, rapid and accurate detection methods for SRBSDV are urgently needed. Recombinase polymerase amplification (RPA) is a novel technique for rapid and sensitive detection of nucleic acids. In this research, a reverse transcription (RT)-RPA-based method was developed for the detection of SRBSDV. A pair of RPA primers targeting the conserved sequences within the SP10 (major coat protein) gene on genomic RNA S10 of SRBSDV were designed. The assay was performed in a single tube at 39 °C for 20 min and demonstrated that the RPA assay is an efficient alternative for rapid detection of SRBSDV.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0166093422000143; http://dx.doi.org/10.1016/j.jviromet.2022.114467; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85122923921&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/35033578; https://linkinghub.elsevier.com/retrieve/pii/S0166093422000143; https://dx.doi.org/10.1016/j.jviromet.2022.114467
Elsevier BV
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