Evaluation of a simple ultrafiltration method for concentration of infective canine parvovirus and feline coronavirus from cell culture supernatants
Journal of Virological Methods, ISSN: 0166-0934, Vol: 310, Page: 114628
2022
- 3Citations
- 8Captures
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Article Description
Enrichment of viral infectious titers following its propagation by cell culture is desirable for various experimental studies. The performance of an ultrafiltration (UF) process to concentrate infectious titers of non-enveloped Canine parvovirus 2 (CPV-2) and enveloped Feline coronavirus (FCoV) obtained from cell culture supernatants was evaluated in this study, and compared with ultracentrifugation (UC) process. A mean gain of > 1.0 log 10 TCID 50 /mL was obtained for CPV-2 with UF, which was comparable with the gain obtained by UC. On the other hand, the gain was lower (0.7–1.0 log 10 TCID 50 /mL) for FCoV with UF in contrast to UC (> 2.0 log 10 TCID 50 /mL). However, the lower retentate volume following UC (∼120 fold) compared to that following UF (∼10 fold) for either of the viruses suggests a trend of increased infectious titer retention in UF concentrates relative to UC concentrates. The simplistic UF process evaluated here thus has the potential for use in applications requiring increased infectious titers of CPV-2 and FCoV.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0166093422001768; http://dx.doi.org/10.1016/j.jviromet.2022.114628; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85139733249&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/36209765; https://linkinghub.elsevier.com/retrieve/pii/S0166093422001768; https://dx.doi.org/10.1016/j.jviromet.2022.114628
Elsevier BV
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